Hormone-sensitive Lipase/HSL [p Ser855] Antibody

Images

 
Western Blot: Hormone-sensitive Lipase/HSL [p Ser855] Antibody [NB100-92690] - Analysis in extracts from HeLa cells.
Immunohistochemistry: Hormone-sensitive Lipase/HSL [p Ser855] Antibody [NB100-92690] - p-HSL (S855) antibody in paraffin-embedded human colon carcinoma tissue.

Order Details


    • Catalog Number
      NB100-92690
    • Availability
      Product Discontinued

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Hormone-sensitive Lipase/HSL [p Ser855] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human HSL around the phosphorylation site of Serine 855.
Modification
p Ser855
Specificity
It has been determined that this antibody is specific to p Ser855.
Clonality
Polyclonal
Host
Rabbit
Gene
LIPE
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of p-HSL (S855) pAb in extracts from HeLa cells treated with Paclitaxel.
Immunohistochemistry (IHC) analyzes of p-HSL (S855) pAb in paraffin-embedded human colon carcinoma tissue.
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Hormone-sensitive Lipase/HSL [p Ser855] Antibody

  • hormone-sensitive lipase testicular isoform
  • Hormonesensitive Lipase
  • Hormone-sensitive Lipase
  • HSL
  • HSLEC 3.1.1.79
  • LHS
  • lipase, hormone-sensitive
  • LIPE

Background

HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LIPE
Entrez