Western blotting (0.5-2 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~32-35 kDa Heme-Oxygenase-1. Jurkat cell lysate can be used as a positive control.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 0.5% BSA and 30% Glycerol
Immunogen affinity purified
Alternate Names for HO-1/HMOX1/HSP32 Antibody
heat shock protein, 32-kD
heme oxygenase (decycling) 1
HO-1heme oxygenase 1
Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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