HO-1/HMOX1/HSP32 Antibody

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Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-45616] - Analysis of HO-1 expression in Jurkat cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, Mk, Pm, RbSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HO-1/HMOX1/HSP32 Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid near the N-terminus of human HO-1.
Marker
Oxidative Stress Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Western blotting (0.5-2 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~32-35 kDa Heme-Oxygenase-1. Jurkat cell lysate can be used as a positive control.
Publications
Read Publication using NBP1-45616.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody

  • bK286B10
  • EC 1.14.99.3
  • heat shock protein, 32-kD
  • heme oxygenase (decycling) 1
  • HMOX1
  • HO
  • HO1
  • HO-1
  • HO-1heme oxygenase 1
  • HSP32

Background

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HO-1/HMOX1/HSP32 Antibody (NBP1-45616)(1)

We have publications tested in 1 confirmed species: Rat.


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Bioinformatics

Gene Symbol HMOX1
Entrez
Uniprot