hnRNP M Antibody

Images

 
Western Blot: hnRNP M Antibody [NBP1-00878] - Western blot (WB) analysis of hn RNP M(P43) antibody in extracts from HT-29 cells.
Immunohistochemistry-Paraffin: hnRNP M Antibody [NBP1-00878] - Analyzes of hnRNP M paraffin-embedded human breast carcinoma tissue at 1:100.
Immunohistochemistry: hnRNP M Antibody [NBP1-00878] - Immunohistochemistry (IHC) analyzes of hnRNP M (P43) antibody in paraffin-embedded human breast carcinoma tissue

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00878
    • Availability
      Product Discontinued

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hnRNP M Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 20-70 of Human hnRNP M.
Clonality
Polyclonal
Host
Rabbit
Gene
HNRNPM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of hn RNP M(P43) pAb in extracts from HT-29 cells.
Immunohistochemistry (IHC) analyzes of hnRNP M (P43) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for hnRNP M Antibody

  • DKFZp547H118
  • heterogeneous nuclear ribonucleoprotein M
  • hnRNP M
  • HNRNPM4
  • HNRPM4heterogenous nuclear ribonucleoprotein M
  • HNRPMheterogenous nuclear ribonucleoprotein M4
  • HTGR1
  • M4 protein
  • N-acetylglucosamine receptor 1
  • NAGR1hnRNA-binding protein M4

Background

Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogenous nuclear ribonucleoproteins (hnRNPs), play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64 kDa subunit of cleavage stimulation factor, which is involved in 3'-end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HNRNPM
Entrez
Uniprot