hnRNP F Antibody (5F5)

Images

 
Western Blot: hnRNP F Antibody (5F5) [NBP2-02221] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY hnRNP F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP F Antibody (5F5) [NBP2-02221] Staining of COS7 cells transiently transfected by pCMV6-ENTRY hnRNP F.
Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Human prostate tissue using anti-hnRNP F mouse monoclonal antibody.
Flow Cytometry: hnRNP F Antibody (5F5) [NBP2-02221] - Analysis of Hela cells, using anti-hnRNP F antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunocytochemistry/ Immunofluorescence: hnRNP F Antibody (5F5) [NBP2-02221] - Immunofluorescent staining of HepG2 cells using anti-hnRNP F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-hnRNP F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-hnRNP F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Human Kidney tissue using anti-hnRNP F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Human liver tissue using anti-hnRNP F mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
5F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

hnRNP F Antibody (5F5) Summary

Immunogen
Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HNRNPF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for hnRNP F Antibody (5F5)

  • heterogeneous nuclear ribonucleoprotein F
  • hnRNP F
  • HNRPFHnRNP F protein
  • mcs94-1
  • MGC110997
  • Nucleolin-like protein mcs94-1
  • OK/SW-cl.23

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HNRNPF
Entrez