HNF-6/ONECUT1 Antibody (4F12)

Images

 
Western Blot: HNF-6/ONECUT1 Antibody (4F12) [NBP1-47903] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: HNF-6/ONECUT1 Antibody (4F12) [NBP1-47903] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HNF6.
Immunohistochemistry-Paraffin: HNF-6/ONECUT1 Antibody (4F12) [NBP1-47903] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HNF6 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
4F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HNF-6/ONECUT1 Antibody (4F12) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 85-198 of human HNF6
Specificity
This antibody is specific for Homo sapiens one cut homeobox 1 (ONECUT1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ONECUT1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HNF-6/ONECUT1 Antibody (4F12)

  • HNF6
  • HNF-6
  • HNF-6A
  • HNF6Ahepatocyte nuclear factor 6, alpha
  • HNF6hepatocyte nuclear factor 6
  • HNF-6One cut domain family member 1
  • OC-1
  • one cut homeobox 1one cut domain, family member 1
  • ONECUT1

Background

The protein encoded by HNF6 is Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a varietyof gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ONECUT1
Entrez
Uniprot