HNF-3 beta/FoxA2 Antibody (3C10)

Images

 
Western Blot: HNF-3 beta/FoxA2 Antibody (3C10) [NBP2-02088] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti HNF-3 beta/FoxA2 monoclonal antibody.(1:200).
Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (3C10) [NBP2-02088] - Staining of HepG2 cells using anti HNF-3 beta/FoxA2 mouse monoclonal antibody at 1:50 dilution.
Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (3C10) [NBP2-02088] - Staining of paraffin-embedded lung using anti-FOXA2 mouse monoclonal antibody.
Flow Cytometry: HNF-3 beta/FoxA2 Antibody (3C10) [NBP2-02088] - HEK293T cells transfected with either pCMV6-ENTRY FOXA2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-FOXA2 mouse monoclonal, ...read more
Western Blot: HNF-3 beta/FoxA2 Antibody (3C10) [NBP2-02088] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (3C10) [NBP2-02088] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2.
Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (3C10) [NBP2-02088] - Staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
3C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HNF-3 beta/FoxA2 Antibody (3C10) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 300-470 of human FOXA2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOXA2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for HNF-3 beta/FoxA2 Antibody (3C10)

  • forkhead box A2
  • Forkhead box protein A2
  • FoxA2
  • hepatic nuclear factor-3-beta
  • hepatocyte nuclear factor 3, beta
  • hepatocyte nuclear factor 3-beta
  • HNF3 beta
  • HNF-3 beta
  • HNF-3B
  • HNF-3-beta
  • HNF3BTCF-3B
  • MGC19807
  • TCF3B
  • Transcription factor 3B

Background

FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FOXA2
Entrez