HMGA1 Antibody

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Western Blot: HMGA1 Antibody [NBP1-36744] - Analysis of HMGA1 in Human Pancreas lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using this antibody at 0.5 ug/ml. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HMGA1 Antibody Summary

Immunogen
Peptide with sequence C-SQPLASKQEKDGT corresponding to internal region (near N-Terminus) according to NP_002122.1.
Epitope
internal region (near N Terminus)
Specificity
This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HMGA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 24 kDa band observed in human pancreas (calculated MW of 11.7 kDa/10.7 kDa band according to NP_665906.1; NP_002122.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. This band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-36744.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HMGA1 Antibody

  • high mobility group AT-hook 1
  • High mobility group AT-hook protein 1
  • High mobility group protein A1
  • high mobility group protein HMG-I/HMG-Y
  • High mobility group protein R
  • high-mobility group (nonhistone chromosomal) protein isoforms I and Y
  • HMGA1A
  • HMG-I(Y)
  • HMGIYMGC12816
  • HMG-R
  • MGC4242
  • MGC4854
  • nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y

Background

This antibody is expected to recognise isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HMGA1 Antibody (NBP1-36744)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HMGA1
Entrez
Uniprot