HLTF Antibody

Images

 
Western Blot: HLTF Antibody [NB100-261] - Analysis of HLTF on HeLa nuclear extract.
Simple Western: HLTF Antibody [NB100-261] - Simple Western lane view shows a specific band for HLTF in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Product Discontinued
View other related HLTF Primary Antibodies

Order Details


    • Catalog Number
      NB100-261
    • Availability
      Product Discontinued

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HLTF Antibody Summary

Immunogen
Synthetic peptide of the human HLTF protein, amino acids 50-292. [UniProt# Q14527]
Clonality
Polyclonal
Host
Rabbit
Gene
HLTF
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Simple Western 1:50
  • Immunoprecipitation 1:10-1:500
Application Notes
This HLTF antibody is useful for Immunoprecipitation and Western Blot, where a band can be seen at approx. 110 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HLTF Lysate (NBL1-11599)

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for HLTF Antibody

  • DNA-binding protein/plasminogen activator inhibitor 1 regulator
  • EC 3.6.1
  • EC 3.6.4.-
  • EC 6.3.2.-
  • helicase-like transcription factor
  • HIP116ARING finger protein 80
  • HIP116matrix associated, actin dependent regulator of chromatin
  • RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3
  • subfamily a, member 3
  • Sucrose nonfermenting protein 2-like 3
  • ZBU1

Background

HLTF (helicase-like transcription factor) belongs to mating-type switching/sucrose non-fermenting (SWI/SNF) family which is implicated in chromatin remodeling and involves in cancer progression either through epigenetic silencing by DNA methylation or through overexpression. Human HLTF shares several structural/functional similarities with yeast RAD5 and a reduction in HLTF expression enhances DNA damage sensitivity and promotes GCR upon DNA damage, and HLTF is able to partially complement for yeast RAD5 function in a sensitized genetic background. HLTF has ubiquitin E3 ligase activity which enhances the polyubiquitination of PCNA in collaboration with RAD6-RAD18. HLTF depletion significantly reduces polyubiquitination of PCNA upon treatment of cells with DNA-damaging agents and HLTF-deficient mouse embryonic fibroblasts exhibit elevated chromosome breaks/fusions after DNA-damaging agent treatment. HLTF is tightly regulated by the ubiquitinproteasome system and HLTF ubiquitination can be catalyzed by CHFR. HLTF is candidate tumor suppressor gene and is a common target for methylation/epigenetic gene silencing in digestive tract cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for HLTF Antibody (NB100-261)

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Diseases for HLTF Antibody (NB100-261)

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Bioinformatics

Gene Symbol HLTF
Entrez
Uniprot