HLCS Antibody (1E4)

Images

 
Western Blot: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: HLCS Antibody (1E4) [NBP2-01859] Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HLCS mouse monoclonal antibody.
Flow Cytometry: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HLCS antibody, and then analyzed by ...read more
Immunohistochemistry-Paraffin: HLCS Antibody (1E4) [NBP2-01859] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HLCS mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
1E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HLCS Antibody (1E4) Summary

Immunogen
Full length human recombinant protein of human HLCS(NP_000402) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLCS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HLCS Antibody (1E4)

  • Biotin apo-protein ligase
  • biotin--protein ligase
  • EC 6.3.4.-
  • HCSholocarboxylase synthetase, EC 6.3.410holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)) ligase)
  • holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase(ATP-hydrolysing)) ligase)
  • holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)] ligase)

Background

This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HLCS
Entrez