Flow Cytometry: HLA ABC Antibody (W6/32) [FITC] [NBP2-00438] - Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple ...read more
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HLA ABC Antibody (W6/32) [FITC] Summary
Purified human tonsil lymphocyte membranes.
NB100-64775 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Protein G purified
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Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
Protein G purified
Alternate Names for HLA ABC Antibody (W6/32) [FITC]
A-10 alpha chain
HLA class I histocompatibility antigen, A-1 alpha chain
HLA class I histocompatibility antigen, A-28 alpha chain
HLA class I histocompatibility antigen, A-9 alpha chain
major histocompatibility complex, class I, A
MHC class I antigen A*1
MHC class I antigen A*11
MHC class I antigen A*80
The W6/32 clone reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. The W6/32 clone recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains (please see our unconjugated antibody for this application).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for HLA ABC Antibody (NBP2-00438). (Showing 1 - 1 of 1 FAQs).
I am trying to perform immunoaffinity chromatography using this antibody, but have been unsuccessful in protein purification. I would like to know whether this antibody is useful for immunoprecipitation and would also like to know to the molecular weight of the antibody?
This antibody has not been validated for immunoprecipitation. We have only validated it for use in flow cytometry. I would not think this antibody would be a good candidate for immunoaffinity purification as it has a fluorescent tag attached. The molecular weight of the antibody is approximately 150kDa (about 50kDa each heavy chain, about 25kDa each light chain). I noticed that the specificity section of this product describes: "W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains." This is in reference to our unconjugated antibody of the same clone W6/32 NB100-64775. This antibody has been validated for IP among other applications. I have updated the datasheet to avoid this confusion again, and changes should be visible on the website in a couple of days.
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