Histone H2B Antibody

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Immunohistochemistry-Paraffin: Histone H2B Antibody [NBP1-50238] - Analysis of anti-Histone H2B antibody with human breast at dilution 1:100.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-50238
    • Availability
      Product Discontinued

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Histone H2B Antibody Summary

Immunogen
KLH-conjugated, synthetic peptide APAPKAcKGSKKAITK corresponding to 7-20 amino acid of human Histone H2B member F. The immunizing sequence has 13 out of 14 identical amino acids in mouse, chicken and rat.
Specificity
Recognizes human acetylated Histone 2B, Mr 15kD. Species sequence identity: mouse, chicken and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H2BF
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • Immunohistochemistry-Paraffin 1:100

Reactivity Notes

Human, Chicken, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera with 30% Glycerol
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Histone H2B Antibody

  • FLJ35099
  • FLJ56780
  • FLJ56787
  • HIST1H2BK
  • histone 2, H2bf
  • histone cluster 2, H2bf
  • histone H2B type 2-F
  • Histone H2B
  • MGC131639

Background

Changes in chromatin structure play a key role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is acetylated in the N-terminal region, primary at lysines 5, 12, 15, and 20 (3). Ubiquitylation of histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of cell apoptosis and meiosis (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HIST2H2BF
Entrez