Histamine H2 R Antibody

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Histamine H2 R Antibody Summary

Immunogen
Peptide with sequence C-QEEKPLKLQVWSGTE corresponding to C-Terminus according to NP_071640.1, NP_001124527.1.
Specificity
This antibody is expected to recognise both reported isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HRH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 0.3 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave bands at approx. 55 kDa band and a stronger 28 kDa band in human colon and duodenum lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 40.1 kDa band according to NP_071640.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC:IF successful usage on coronal rat brain slices is reported in scientific literature (PMID: 23466693).
Control Peptide
Histamine H2 R Peptide (NB600-812PEP)
Publications
Read Publication using NB600-812.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Mouse, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Histamine H2 R Antibody

  • Gastric Receptor 1
  • Gastric receptor I
  • H2R
  • HH2R
  • Histamine H2 R
  • histamine H2 receptor
  • Histamine H2R
  • histamine receptor H2
  • HRH2

Background

This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histamine H2 R Antibody (NB600-812)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HRH2
Entrez
Uniprot