HIST1H3A [Dimethyl Lys27] Antibody

Images

 
Western Blot: HIST1H3A [Dimethyl Lys27] Antibody [NBP2-41666] - HeLa cells extracts (15 ug) were analysed by WB blot using the antibody.
Chromatin Immunoprecipitation (ChIP): HIST1H3A [Dimethyl Lys27] Antibody [NBP2-41666] - A titration of the antibody consisting of 1, 2, 5, and 10 ul per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative ...read more
ELISA: HIST1H3A [Dimethyl Lys27] Antibody [NBP2-41666] - By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:480,000.
Dot Blot: HIST1H3A [Dimethyl Lys27] Antibody [NBP2-41666] - Analysis was performed to test the cross reactivity of the antibody with peptides containing other modifications of histone H3 and the unmodified H3K9 ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-41666
    • Availability
      Product Discontinued

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HIST1H3A [Dimethyl Lys27] Antibody Summary

Immunogen
KLH-conjugated synthetic peptide of Histone H3 containing dimethylated lysine 27.
Modification
Dimethyl Lys27
Clonality
Polyclonal
Host
Rabbit
Gene
HIST1H3A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation
  • Dot Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
WB: 1:1000, ELISA: 1:1000, Dot Blot: 1:50,000, IF: 1:500, ChIP: 2 ug/ChIP.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide and 0.05% ProClin 300
Purity
Immunogen affinity purified

Alternate Names for HIST1H3A [Dimethyl Lys27] Antibody

  • H3 histone family, member A
  • H3/A
  • H3FAHIST1H3E
  • H3FB
  • H3FC
  • H3FD
  • H3FF
  • H3FH
  • H3FI
  • H3FJ
  • H3FK
  • H3FL
  • HIST1H3B
  • HIST1H3C
  • HIST1H3D
  • HIST1H3F
  • HIST1H3G
  • HIST1H3H
  • HIST1H3I
  • HIST1H3J
  • histone 1, H3a
  • histone cluster 1, H3a
  • histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/i
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HIST1H3A
Uniprot