His Tag Antibody

Images

 
Western Blot: 6-Histidine Epitope Tag Antibody [NBP1-51166] - Analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:2000 (lane 1) and 1:5000 (lane 2). Each lane was loaded with 10 ug of ...read more
Western Blot: 6-Histidine Epitope Tag Antibody [NBP1-51166] - Extracts from HEK293A cells,transfected with His-tag vector.

Product Details

Summary
Product Discontinued
View other related His Tag Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51166
    • Availability
      Product Discontinued

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His Tag Antibody Summary

Immunogen
Synthetic peptide His-tag
Specificity
His-tag pAb detects over-expressed or recombinant proteins containing the 6x His epitope tag
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
Application Notes
Western blot (WB) analyzes of His-tag pAb in His-tagged protein purified from E.coli. Use in ELISA reported in scientific literature (PMID 24300951)
Publications
Read Publications using NBP1-51166.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for His Tag Antibody

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for His Tag Antibody (NBP1-51166)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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