Western Blot: 6-Histidine Epitope Tag Antibody [HRP] [NBP1-42785] - Detection of1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein ...read more
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
FAQs for His Tag Antibody (NBP1-42785). (Showing 1 - 1 of 1 FAQs).
What is the time of incubation for 6-histidine epitope tag antibody (HRP)?
I am not sure what application you are asking about, as NBP1-42785 is guaranteed for use in both Western blot and IHC on paraffin-embedded tissues. Generally we recommend overnight at 4 degrees C for both Western and IHC. As this is an antibody to simply a His-tag, you can probably get away with incubating for an hour or two at room temperature as well.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.