His Tag Antibody [HRP]

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Western Blot: 6-Histidine Epitope Tag Antibody [HRP] [NBP1-42785] - Detection of1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42785
    • Availability
      Product Discontinued

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His Tag Antibody [HRP] Summary

Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:5000
  • ELISA 1:5000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
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Reviewed Applications
Read 1 Review rated 5
using
NBP1-42785 in the following applications:

Publications
Read Publications using NBP1-42785.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water (or equivalent) to desired concetration.

Alternate Names for His Tag Antibody [HRP]

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for His Tag Antibody (NBP1-42785)(3)

Review for His Tag Antibody (NBP1-42785) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-42785:
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 11/21/2011
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Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
CommentsGood! A single band was detected as expected

Blocking

Blocking DetailsBlocking Buffer: 5% skim milk in PBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:2000, Primary Ab Incubation Time: 2 hours

Details

Detection NotesDetection Method: ECL

Comments

CommentsGood! A single band was detected as expected

Product General Protocols

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WB Video Protocol

FAQs for His Tag Antibody (NBP1-42785). (Showing 1 - 1 of 1 FAQs).

  1. What is the time of incubation for 6-histidine epitope tag antibody (HRP)?
    • I am not sure what application you are asking about, as NBP1-42785 is guaranteed for use in both Western blot and IHC on paraffin-embedded tissues. Generally we recommend overnight at 4 degrees C for both Western and IHC. As this is an antibody to simply a His-tag, you can probably get away with incubating for an hour or two at room temperature as well.

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Anonymous
11/21/2011
Application: WB
Species: Mouse