His Tag Antibody (BV-G020)

Images

 
Western Blot: 6-Histidine Epitope Tag Antibody (BV-G020) [NBP1-50707] - 393 cells transfected with His-tagged vector (Lane 2) and control vector (Lane 1).
Immunocytochemistry/ Immunofluorescence: 6-Histidine Epitope Tag Antibody (BV-G020) [NBP1-50707]

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ICC/IF, IP
Clone
BV-G020
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

His Tag Antibody (BV-G020) Summary

Immunogen
6xHis synthetic peptide
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation
Application Notes
Western blotting (1-2 ug/ml), immunoprecipitation (10-20 ug/ml), and immunocytochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually.

Reactivity Notes

All

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 1.0% BSA and 50% Glycerol
Preservative
0.02% Thimerosal
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for His Tag Antibody (BV-G020)

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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