His Tag Antibody (33D10.D2) [DyLight 488]

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Fluorophore-linked immunosorbent assay: 6-Histidine Epitope Tag Antibody (33D10.D2) [DyLight 488] [NBP1-97329]

Product Details

Summary
Reactivity NASpecies Glossary
Applications ELISA, FLISA, ICC/IF
Clone
33D10.D2
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488
Concentration
LYOPH

Order Details

View Available Conjugates

His Tag Antibody (33D10.D2) [DyLight 488] Summary

Immunogen
This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Fluorophore-linked immunosorbent assay 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Reactivity Notes

6x His tag specific

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water to a final concentration of 1.0 mg/ml.

Notes

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free Color: Green Excitation Wavelength: 493 Emission Wavelength: 518 Conjugation Chemistry: N-hydroxysuccinimide (NHS) ester. Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for His Tag Antibody (33D10.D2) [DyLight 488]

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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