HIPPI Antibody (4F6)

Images

 
Western Blot: HIPPI Antibody (4F6) [NBP2-02313] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIPPI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: HIPPI Antibody (4F6) [NBP2-02313] Staining of COS7 cells transiently transfected by pCMV6-ENTRY HIPPI.
Immunohistochemistry-Paraffin: HIPPI Antibody (4F6) [NBP2-02313] - Staining of paraffin-embedded Kidney tissue using anti-HIPPI mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HIPPI Antibody (4F6) [NBP2-02313] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-HIPPI mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HIPPI Antibody (4F6) [NBP2-02313] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-HIPPI mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HIPPI Antibody (4F6) [NBP2-02313] - Staining of paraffin-embedded breast tissue using anti-HIPPI mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
4F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HIPPI Antibody (4F6) Summary

Immunogen
Full length human recombinant protein of human IFT57(NP_060480) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IFT57
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HIPPI Antibody (4F6)

  • Dermal papilla-derived protein 8
  • DERP8
  • ESRRBL1huntingtin interacting protein-1 interacting protein
  • estrogen-related receptor beta like 1
  • Estrogen-related receptor beta-like protein 1
  • FLJ10147
  • HIPPIHIP1-interacting protein
  • intraflagellar transport 57 homolog (Chlamydomonas)
  • intraflagellar transport protein 57 homolog
  • MHS4R2HIP1 protein interactor

Background

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1,leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3'present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IFT57
Entrez