HIP2 Antibody

Images

 
Western Blot: HIP2 Antibody [NBP2-16831] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HIP2 Antibody [NBP2-16831] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: HIP2 Antibody [NBP2-16831] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HIP2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HIP2. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2K
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HIP2 Antibody

  • DKFZp564C1216
  • DKFZp686J24237
  • E2(25K)
  • E2-25K
  • EC 6.3.2.19
  • HIP-2
  • HIP2LIG
  • huntingtin interacting protein 2
  • Huntingtin-interacting protein 2
  • HYPG
  • Ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2 K
  • Ubiquitin-conjugating enzyme E2(25K)
  • Ubiquitin-conjugating enzyme E2-25 kDa
  • Ubiquitin-conjugating enzyme E2-25K
  • ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
  • Ubiquitin-protein ligase

Background

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBE2K
Uniprot