HIP2 Antibody

Images

 
Western Blot: HIP2 Antibody [NB300-840] - (1.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: HIP2 Antibody [NB300-840] - (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6,

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HIP2 Antibody Summary

Immunogen
Peptide with sequence C-SWDVETATELLLSN corresponding to C-Terminus according to NP_005330.1, NP_001104582.1, NP_001104583.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UBE2K
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 24 kDa band observed in Jurkat lysates (calculated MW of 22.4 kDa band according to NP_005330). IHC-P: Human brain shows pixulate cytoplasm stainng in neurons of the cortex.
Positive Control
HIP2 Lysate (NBL1-17536)
Publications
Read Publication using NB300-840.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HIP2 Antibody

  • DKFZp564C1216
  • DKFZp686J24237
  • E2(25K)
  • E2-25K
  • EC 6.3.2.19
  • HIP-2
  • HIP2LIG
  • huntingtin interacting protein 2
  • Huntingtin-interacting protein 2
  • HYPG
  • Ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2 K
  • Ubiquitin-conjugating enzyme E2(25K)
  • Ubiquitin-conjugating enzyme E2-25 kDa
  • Ubiquitin-conjugating enzyme E2-25K
  • ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
  • Ubiquitin-protein ligase

Background

HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequent degradation of p53. Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIP2 Antibody (NB300-840)(1)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBE2K
Entrez
Uniprot