HIN-1/SCGB3A1 Antibody

Western blot shows lysates of rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat HIN-1/SCGB3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIN-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HIN-1/SCGB3A1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse HIN‑1/SCGB3A1
  Phe22-Gly104
  Accession # Q920D7
• Specificity: Detects mouse HIN‑1/SCGB3A1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human HIN-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Otud4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HIN-1/SCGB3A1 Antibody

• cytokine high in normal-1
• Cytokine HIN-1
• High in normal 1
• HIN1
• HIN-1
• HIN1Pneumo secretory protein 2
• LU105
• PNSP2
• PnSP-2
• PnSP-2Uteroglobin-related protein 2
• SCGB3A1
• secretoglobin family 3A member 1
• secretoglobin, family 3A, member 1
• UGRP2
• UGRP2MGC87867

Background

HIN-1, also known as PnSP‑2, LuLeu‑2, UGRP‑2 and SCGB3A1, is an 10 kDa member of the secretoglobulin family of molecules. It is synthesized as a 104 amino acid (aa) precursor that contains a 21 aa signal sequence and an 83 aa mature region. There are no potential N‑linked glycosylation sites. Based on other members of the family, HIN‑1 is likely to exist as a disulfide‑linked homodimer. The molecule is found in mammary and upper airway epithelium. No receptor for HIN‑1 has been identified. Mature mouse HIN‑1 shares 60% aa sequence identity with mature human HIN‑1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIN-1/SCGB3A1 Antibody (AF2954)(3)

Publications using AF2954

• Xu Naizhen, Taketomo Kido, Shigetoshi Yokoyama, R. Ilona Linnoila, Shioko Kimura Spatiotemporal Expression of Three Secretoglobin Proteins, SCGB1A1, SCGB3A1, and SCGB3A2, in Mouse Airway Epithelia Journal of Histochemistry & Cytochemistry 6/1/2019 [PMID: 30768367]
• Thomas M. Raffay, Morgan L. Locy, Cynthia L. Hill, Nik S. Jindal, Lynette K. Rogers, Stephen E. Welty et al. Neonatal Hyperoxic Exposure Persistently Alters Lung Secretoglobins and Annexin A1 BioMed Research International 9/26/2013 [PMID: 24187664]
• Yamada A, Suzuki D, Miyazono A, Oshima K, Kamiya A, Zhao B, Takami M, Donnelly RP, Itabe H, Yamamoto M, Kimura S, Kamijo R IFN-gamma down-regulates Secretoglobin 3A1 gene expression. Biochem. Biophys. Res. Commun., 2009-01-09;379(4):964-8. 2009-01-09 [PMID: 19135978] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Otud4
• Uniprot:
  • Q920D7()