HIF-3 alpha Overexpression Lysate (Denatured)

Images

 
Lane 1: HIF3A transfected lysate (73.7 KDa) Lane 2: Non-transfected lysate.
HIF3A transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HIF-3 alpha Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-HIF3A full-length
Gene
HIF3A

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for HIF-3 alpha

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-HIF3A antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HIF-3 alpha Overexpression Lysate (Denatured)

  • Basic-helix-loop-helix-PAS protein MOP7
  • BHLHE17
  • bHLHe17HIF-3A2
  • Class E basic helix-loop-helix protein 17
  • HIF-3A4
  • HIF-3-alpha
  • HIF3-alpha
  • hypoxia inducible factor 3, alpha subunit
  • hypoxia-inducible factor 3-alpha
  • hypoxia-inducible factor-3 alpha 4
  • Inhibitory PAS domain protein
  • IPASHIF3-alpha-1
  • Member of PAS protein 7
  • MOP7PAS domain-containing protein 7
  • PASD7HIF-3A

Background

The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HIF-3 alpha Lysate (H00064344-T02) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HIF-3 alpha Lysate (H00064344-T02). (Showing 1 - 1 of 1 FAQ).

  1. I'm testing HIF-3A antibody (NB100-2529). I have query about its specificity and expected size: mostly I see band at about 40 kDa instead of 71 (the samples are proteins from cell cultures grown in hypoxic conditions). In your control HIF-3A lysates one H00064344-T02 detecting band at 73 kDa and other H00064344-T01 at 37kDa. Since I'm running the SDS-PAGE, the band at 40kDa might be correct?
    • This protein has multiple isoforms (7) and they might have different expression patterns in various cell lines and tissue. As far as our overexpression control lysates are concerned, H00064344-T02 detects an isoform which is 72kd whereas in H00064344-T01 most probably isoform 4 which is around 40 KD is detected. It is also important to keep in mind that isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secures an inhibition of HIF1A activity. Please see this UniProt link for more information on HIF3A.

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Blogs on HIF-3 alpha.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF Prolyl Hydroxylase 2: an important Oxygen Sensor Protein
Prolyl hydroxylase domain (PHD) proteins, including PHD1, PHD2, and PHD3, mediate oxygen-dependent degradation of hypoxia-inducible factor (HIF) alpha subunits. Suppression of PHD enzymes leads to stabilization of HIFs and offers a potential treatment...  Read full blog post.

HIF Antibodies: Beyond HIF-1 alpha
The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the...  Read full blog post.

A Role for HIF-1 alpha Antibody in Renal Research
The Hypoxia Inducible Factors (HIFs) are a family of mammalian transcription factors which are expressed in response to low cellular oxygen concentrations (hypoxia). Three human hypoxia inducible factors have been identified, HIF-1, HIF-2 and HIF-3, e...  Read full blog post.

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Bioinformatics

Gene Symbol HIF3A