HIF-3 alpha Overexpression Lysate (Denatured)


Lane 1: HIF3A transfected lysate (73.7 KDa) Lane 2: Non-transfected lysate.
HIF3A transfected lysate.

Product Details

Reactivity HuSpecies Glossary
Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

HIF-3 alpha Overexpression Lysate (Denatured) Summary

hypoxia inducible factor 3, alpha subunit

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
50mM Tris-HCl, 2%SDS, 10% glycerol, 2% 2-mercaptoethanol, pH 6.8.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This transient overexpression cell lysate is useful for western blot and was tested with Anti-HIF3A antibody H00064344-B02 by Western Blots.

Lysate Details for HIF-3 alpha

Protein State

Reactivity Notes



This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HIF-3 alpha Overexpression Lysate (Denatured)

  • Basic-helix-loop-helix-PAS protein MOP7
  • BHLHE17
  • bHLHe17HIF-3A2
  • Class E basic helix-loop-helix protein 17
  • HIF-3A4
  • HIF-3-alpha
  • HIF3-alpha
  • hypoxia inducible factor 3, alpha subunit
  • hypoxia-inducible factor 3-alpha
  • hypoxia-inducible factor-3 alpha 4
  • Inhibitory PAS domain protein
  • IPASHIF3-alpha-1
  • Member of PAS protein 7
  • MOP7PAS domain-containing protein 7


The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HIF-3 alpha Lysate (H00064344-T02). (Showing 1 - 1 of 1 FAQ).

  1. I'm testing HIF-3A antibody (NB100-2529). I have query about its specificity and expected size: mostly I see band at about 40 kDa instead of 71 (the samples are proteins from cell cultures grown in hypoxic conditions). In your control HIF-3A lysates one H00064344-T02 detecting band at 73 kDa and other H00064344-T01 at 37kDa. Since I'm running the SDS-PAGE, the band at 40kDa might be correct?
    • This protein has multiple isoforms (7) and they might have different expression patterns in various cell lines and tissue. As far as our overexpression control lysates are concerned, H00064344-T02 detects an isoform which is 72kd whereas in H00064344-T01 most probably isoform 4 which is around 40 KD is detected. It is also important to keep in mind that isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secures an inhibition of HIF1A activity. Please see this UniProt link for more information on HIF3A.

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Blogs on HIF-3 alpha.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF Prolyl Hydroxylase 2: an important Oxygen Sensor Protein
Prolyl hydroxylase domain (PHD) proteins, including PHD1, PHD2, and PHD3, mediate oxygen-dependent degradation of hypoxia-inducible factor (HIF) alpha subunits. Suppression of PHD enzymes leads to stabilization of HIFs and offers a potential treatment...  Read full blog post.

HIF Antibodies: Beyond HIF-1 alpha
The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the...  Read full blog post.

A Role for HIF-1 alpha Antibody in Renal Research
The Hypoxia Inducible Factors (HIFs) are a family of mammalian transcription factors which are expressed in response to low cellular oxygen concentrations (hypoxia). Three human hypoxia inducible factors have been identified, HIF-1, HIF-2 and HIF-3, e...  Read full blog post.

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Gene Symbol HIF3A

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