hHpr1-p84-Thoc1 Antibody

Images

 
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-47303] - Sample: FFPE section of human ovarian carcinoma. Antibody was used at a dilution of 1:1,000 (1ug/ml).
Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP1-47303] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-47303 used for IP at 3 ug/mg lysate. THOC1 was also immunoprecipitated by ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47303
    • Availability
      Product Discontinued

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hHpr1-p84-Thoc1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 550 and 600 of human THO Complex 1 using the numbering given in entry NP_005122.2 (GeneID 9984).
Clonality
Polyclonal
Host
Rabbit
Gene
THOC1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:1000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for hHpr1-p84-Thoc1 Antibody

  • HPR1THO complex subunit 1
  • hTREX84
  • Nuclear matrix protein p84
  • P84
  • P84N5
  • THO complex 1
  • tho1

Background

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol THOC1
Entrez