HGF R/c-MET [p Tyr1349] Antibody

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Western Blot: HGF R/c-MET [p Tyr1349] Antibody [NBP1-51450] - Analysis of p-Met (Y1349) pAb at 1:500 dilution Lane1:THP-1 cell lysate treated with EGF(0.1ng/ml,30mins) Lane2:Hela cell lysate treated with ...read more
Western Blot: HGF R/c-MET [p Tyr1349] Antibody [NBP1-51450] - Extracts from HepG2 cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51450
    • Availability
      Product Discontinued

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HGF R/c-MET [p Tyr1349] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human c-Met around the phosphorylation site of Tyrosine 1349.
Modification
p Tyr1349
Specificity
p-Met (Y1349) pAb detects endogenous levels of Met protein only when phosphorylated at Y1349.
Clonality
Polyclonal
Host
Rabbit
Gene
MET
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analyzes of p-Met (Y1349) pAb in extracts from HepG2 cells.
Theoretical MW
156 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HGF R/c-MET [p Tyr1349] Antibody

  • AUTS9
  • cMET
  • c-MET
  • EC 2.7.10
  • EC 2.7.10.1
  • hepatocyte growth factor receptor
  • HGF R
  • HGF receptor
  • HGF/SF receptor
  • HGFR
  • Met (c-Met)
  • met proto-oncogene (hepatocyte growth factor receptor)
  • met proto-oncogene tyrosine kinase
  • MET
  • oncogene MET
  • Proto-oncogene c-Met
  • RCCP2
  • Scatter factor receptor
  • SF receptor
  • Tyrosine-protein kinase Met

Background

The c-Met oncogene was originally isolated from a chemical carcinogen-treated human osteogenic sarcoma cell line by transfection analysis in NIH/3T3 cells. The Met proto-oncogene product was identified as a transmembrane receptorlike protein with tyrosine kinase activity that is expressed in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibit transforming activity.Tyrosine phosphorylation of apparently normal Met protein has also been observed in certain human gastric carcinoma cell lines. Tyrosine phosphorylation enhances the receptor kinase activity, while serine phosphorylation of Met on residue 985 has an inhibitory effect. The c-Met gene product has been identified as the cell surface receptor for hepatocyte growth factor, a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MET
Entrez