Hexokinase 2 Antibody (4C5)

Images

 
Western Blot: Hexokinase 2 Antibody (4C5) [NBP2-02272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hexokinase 2I monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (4C5) [NBP2-02272] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hexokinase 2.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody (4C5) [NBP2-02272] - Staining of paraffin-embedded prostate tissue using anti-Hexokinase 2 mouse monoclonal antibody.
Flow Cytometry: Hexokinase 2 Antibody (4C5) [NBP2-02272] - HEK293T cells transfected with either pCMV6-ENTRY Hexokinase 2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Hexokinase 2 mouse ...read more
Western Blot: Hexokinase 2 Antibody (4C5) [NBP2-02272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hexokinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: Hexokinase 2 Antibody (4C5) [NBP2-02272] - Staining of HepG2 cells using anti-Hexokinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody (4C5) [NBP2-02272] - Staining of paraffin-embedded endometrium tissue using anti-Hexokinase 2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Hexokinase 2 Antibody (4C5) [NBP2-02272] - Staining of paraffin-embedded pancreas tissue using anti-Hexokinase 2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Ca, Mk, Mu, Rt, Po, EqSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
4C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Hexokinase 2 Antibody (4C5) Summary

Immunogen
Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.
Specificity
Human Hexokinase-2 protein is 74% identical with Hexokinase-1 and our Hexokinase 2 antibody (clone 4C5) is not expected to crossreact with Hexokinase-1.
Predicted Species
Mouse (94%), Rat (94%), Porcine (96%), Equine (94%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Hexokinase 2 Antibody (4C5)

  • DKFZp686M1669
  • EC 2.7.1
  • Hexokinase 2
  • Hexokinase type II
  • HK2
  • HKII
  • HXK2
  • Muscle form hexokinase
  • muscle

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways.This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-32920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF4036
Species: Hu
Applications: WB, Simple Western
H00005324-M02
Species: Hu
Applications: WB, ELISA, IHC
NBP1-85789
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB4104
Species: Hu
Applications: WB, IP, Neut
MAB35661
Species: Hu
Applications: WB, IHC, IP, Neut
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-47778
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB2337
Species: Hu
Applications: WB, IP
NB110-39113
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-38799
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-89691
Species: Hu
Applications: IHC, IHC-P
NBP1-81743
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-81745
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NBP2-20881
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Hexokinase 2 Antibody (NBP2-02272) (0)

There are no publications for Hexokinase 2 Antibody (NBP2-02272).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Hexokinase 2 Antibody (NBP2-02272) (0)

There are no reviews for Hexokinase 2 Antibody (NBP2-02272). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Hexokinase 2 Antibody (NBP2-02272) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Hexokinase 2 Products

Bioinformatics Tool for Hexokinase 2 Antibody (NBP2-02272)

Discover related pathways, diseases and genes to Hexokinase 2 Antibody (NBP2-02272). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Hexokinase 2 Antibody (NBP2-02272)

Discover more about diseases related to Hexokinase 2 Antibody (NBP2-02272).
 

Pathways for Hexokinase 2 Antibody (NBP2-02272)

View related products by pathway.

PTMs for Hexokinase 2 Antibody (NBP2-02272)

Learn more about PTMs related to Hexokinase 2 Antibody (NBP2-02272).
 

Research Areas for Hexokinase 2 Antibody (NBP2-02272)

Find related products by research area.

Blogs on Hexokinase 2

There are no specific blogs for Hexokinase 2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Hexokinase 2 Antibody (4C5) and receive a gift card or discount.

Bioinformatics

Gene Symbol HK2
Entrez