HES-1 Antibody (OTI7B9)


Western Blot: Hes1 Antibody (7B9) [NBP2-01595] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hes1 monoclonal antibody.
Immunohistochemistry-Paraffin: Hes1 Antibody (7B9) [NBP2-01595] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Hes1 mouse monoclonal antibody.
Western Blot: Hes1 Antibody (7B9) [NBP2-01595] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hes1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more

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HES-1 Antibody (OTI7B9) Summary

Full length human recombinant protein of human HES1(NP_005515) produced in HEK293T cell.
Immunogen affinity purified
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  • Western Blot 1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human, Monkey, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for HES-1 Antibody (OTI7B9)

  • BHLHB39
  • bHLHb39hairy homolog (Drosophila)
  • Class B basic helix-loop-helix protein 39
  • FLJ20408
  • Hairy and enhancer of split 1
  • hairy and enhancer of split 1, (Drosophila)
  • Hairy homolog
  • Hairy-like protein
  • HES1
  • HES-1
  • hHL
  • HL
  • HRY
  • transcription factor HES-1


This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HES-1 Antibody (NBP2-01595). (Showing 1 - 1 of 1 FAQ).

  1. What is the molecular weight of the band targeted by NBP2-01595 (Hes 1) in WB? The two images showed bands of different location. It's confusing.
    • Yes, you are correct, that WB image from 9 different cell lines is very confusing. I did mention this to our lab and they are going to re-test the antibody in those cell lines. Upon receiving the WB image, I will forward it to you and our team at Novus will update the image on the datasheet as well. I do apologize for the confusion, please feel free to contact me if you have more questions or concerns.

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Gene Symbol HES1