HES-1 Antibody (4H1)

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Western Blot: HES-1 Antibody (4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hes1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HES-1 Antibody (4H1) [NBP1-47791] - Staining of paraffin-embedded liver using anti-Hes1 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (4H1) [NBP1-47791] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hes1.
Immunohistochemistry-Paraffin: HES-1 Antibody (4H1) [NBP1-47791] - Staining of paraffin-embedded breast using anti-Hes1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HES-1 Antibody (4H1) [NBP1-47791] - Staining of paraffin-embedded Carcinoma of liver using anti-Hes1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HES-1 Antibody (4H1) [NBP1-47791] - Staining of paraffin-embedded endometrium using anti-Hes1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HES-1 Antibody (4H1) [NBP1-47791] - Staining of paraffin-embedded kidney using anti-Hes1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P
Clone
4H1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HES-1 Antibody (4H1) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1
Specificity
This antibody is specific for Homo sapiens hairy and enhancer of split 1, (Drosophila) (HES1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HES1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Publications
Read Publications using
NBP1-47791 in the following applications:

  • 3 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for HES-1 Antibody (4H1)

  • BHLHB39
  • bHLHb39hairy homolog (Drosophila)
  • Class B basic helix-loop-helix protein 39
  • FLJ20408
  • Hairy and enhancer of split 1
  • hairy and enhancer of split 1, (Drosophila)
  • Hairy homolog
  • Hairy-like protein
  • HES1
  • HES-1
  • hHL
  • HL
  • HRY
  • HRYHHL
  • transcription factor HES-1

Background

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HES-1 Antibody (NBP1-47791)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ChIP, WB.


Filter By Application
ChIP
(3)
WB
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 5 of 5.
Publications using NBP1-47791 Applications Species
Liao B, Zhou H, Liang H, Li C. Regulation of ERK and AKT pathways by hepatitis B virus X protein via the Notch1 pathway in hepatocellular carcinoma. Int. J. Oncol. 2017 Nov 01 [PMID: 29048612] (ChIP, Human) ChIP Human
Jeong JY, Kang H, Kim TH et al. MicroRNA-136 inhibits cancer stem cell activity and enhances the anti-tumor effect of paclitaxel against chemoresistant ovarian cancer cells by targeting Notch3. Cancer Lett. Nov 22 2016 [PMID: 27887917] (WB, Human) WB Human
Chiou HY,Liu SY,Lin CH, Lee EH. Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45a expression to increase cell survival J. Biomed. Sci. 2014 Jun 26 [PMID: 24894488] (ChIP, Human)

Details:
Hes1 antibody used for Chromatin Immunoprecipitation /ChIP assay in transfected HEK293T cells - nuclear chromatin incubated Hes-1 antibody 4.8 ug or mouse IgG or Flag-M2, 4C ON, immunoprecipitates collected on magnesium beads (1-2 h 4C), immunoprecipitates washed and de-crosslinked, chromatin processed for quantitative PCR analysis (Figure 6B).
ChIP Human
Maraver A, Fernandez-Marcos PJ, Herranz D et al. Therapeutic Effect of Secretase Inhibition in Kras(G12V)-Driven Non-Small Cell Lung Carcinoma by Derepression of DUSP1 and Inhibition of ERK Cancer Cell 2012 Aug 14 [PMID: 22897852] (ChIP, Human) ChIP Human
Wang CY, Wei Q, Han I et al. Hedgehog and Notch signaling regulate self-renewal of undifferentiated pleomorphic sarcomas. Cancer Res 2012 Feb 15 [PMID: 22232736]

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for HES-1 Antibody (NBP1-47791). (Showing 1 - 1 of 1 FAQs).

  1. Can you elaborate in detail regarding the specificity mentioned for NBP1-47791?
    • This antibody has been created to detect the human HES1 protein (UniProt Q14469). The immunogen information given states that the immunogen corresponds to amino acids 1-280 of human HES1, which according to the UniProt entry I've just linked to is the whole protein. I think the mention of Drosophila in the specificity information is referring to the derivation of the name of this protein. Wikipedia says, 'Transcription factor HES1 (hairy and enhancer of split-1) is a protein that is encoded by the Hes1 gene, and is the mammalian homolog of the hairy gene in Drosophila'.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol HES1
Entrez
Uniprot