Hepatitis C Virus Antibody (TORDJI-22)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-65708
    • Availability
      Product Discontinued

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Hepatitis C Virus Antibody (TORDJI-22) Summary

Immunogen
Human plasma membrane
Specificity
This antibody recognises a cytoplasmic antigen of human Hepatitis C virus; it does not appear to cross react with either Hepatitis A or Heptatitis B virus as shown by immunohistochemistry. In Western Blotting NB100-65708 is specific for the HCV NS4 protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunohistochemistry-Paraffin, Immunofluorescence, Western Blot, Immunohistochemistry-Frozen, ELISA. In WB detects a band of approximately 130 kDa in platelet lysates. Megakaryocytes and platelets are labeled in sections of decalcified bone without pretreatment and with pretreatment using protease type XXIV (0.5 mg/ml). HIER (microwave, citrate buffer pH 6.0) may give superior results.

Reactivity Notes

Viral.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for Hepatitis C Virus Antibody (TORDJI-22)

  • HCV NS5
  • Hepatitis C virus non structural protein 5
  • Hepatitis C virus nonstructural protein 5
  • Hepatitis C Virus NS5

Background

HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Hepatitis C Virus Antibody (NB100-65708). (Showing 1 - 1 of 1 FAQ).

  1. We are testing the antibody Hepatitis C Virus Antibody (TORDJI-22) in liver biopsies and we have identified cytoplasmic and nuclear liver cells and lymphocytes immunoreaction. We would like to ask the correct reaction location since we couldn't find any information about it in the datasheet, and mainly known if this immunoreaction found in lymphocytes has a important meaning in the context.
    • This antibody is believed to bind the NS4 protein of HCV and is known to give cytoplasmic staining. Furthermore, HCV is known to infect lymphocytes (Wikipedia), so seeing this reactivity in your samples is to be expected.

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