Recombinant Hepatitis B preS2 Protein

• Applications: ELISA, PAGE
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Hepatitis B preS2 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 55 amino acids corresponding to Hepatitis B preS2 Source: E. coli

  Amino Acid Sequence: MQWNSTTFHQ ALLDPKVRGL YFPAGGSSSG TVNPVPTTAS PISSIFSRTG DPAPN
  
  This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

• Details of Functionality: Data is not available
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Hepatitis B preS2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • SDS-Page
• Theoretical MW: 5.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20mM PB, pH 7.4, 50mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Hepatitis B preS2 Protein

• HBV pre S2 Antigen
• HBV pre S2 protein
• Hepatitis B Virus pre S2 protein
• PreS2/S antigen
• S

Background

Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, MHBs has a 55 amino acid region called preS2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.