Helicobacter Pylori Antibody (51-13)

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Product Details

Summary
Product Discontinued
View other related Helicobacter Pylori Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51725
    • Availability
      Product Discontinued

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Helicobacter Pylori Antibody (51-13) Summary

Immunogen
The antibody reacts specifically with Helicobacter bacteria.
Specificity
The antibody reacts specifically with Helicobacter bacteria. It does not react with other members of the Enterobacteriaceae.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Aggregation
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
The antibody is reactive with helicobacter using ELISA and immunofluorescence on bacteria fixed on a glass slide.For use in immunofluorescence the antibody should be used undiluted, for ELISA users should determine their own optimal working dilutions. For immunofluorescence on bacteria solution, bacteria are spread on a slide and dried. Fix bacteria by 3 fast strokes through gas flame. Incubate with monoclonal, wash and incubate with appropriate fluorescent conjugate.For ELISA prepare bacteria suspension in PBS (5x108 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.

Reactivity Notes

Bacteria

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and 1% BSA.
Preservative
0.1% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Helicobacter Pylori Antibody (51-13)

  • H. pylori

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Helicobacter Pylori Products

Helicobacter Pylori NBP1-51725

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