Helicobacter Pylori Antibody (51-13)



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Helicobacter Pylori Antibody (51-13) Summary

The antibody reacts specifically with Helicobacter bacteria.
The antibody reacts specifically with Helicobacter bacteria. It does not react with other members of the Enterobacteriaceae.
Protein A or G purified
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  • Aggregation
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
The antibody is reactive with helicobacter using ELISA and immunofluorescence on bacteria fixed on a glass slide.For use in immunofluorescence the antibody should be used undiluted, for ELISA users should determine their own optimal working dilutions. For immunofluorescence on bacteria solution, bacteria are spread on a slide and dried. Fix bacteria by 3 fast strokes through gas flame. Incubate with monoclonal, wash and incubate with appropriate fluorescent conjugate.For ELISA prepare bacteria suspension in PBS (5x108 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS and 1% BSA.
0.1% Sodium Azide
Protein A or G purified

Alternate Names for Helicobacter Pylori Antibody (51-13)

  • H. pylori


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Additional Helicobacter Pylori Products

Helicobacter Pylori NBP1-51725

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