HeLa Whole Cell Lysate

Product Discontinued

HeLa Whole Cell Lysate Summary

• Description: Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue. Whole cell lysate (non-denatured)
• Localization: Cervix
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay.

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: In modified RIPA Lysis Buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF).
• Concentration: 5 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Cervix
• Protein State: Native
• Subcellular Fraction: Whole

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

HeLa cells are the oldest and most commonly used human cell line in research. HeLa was derived from cervical cancer cells taken on February 9, 1951 from Henrietta Lacks. They were immortalized by George Otto Gey at Johns Hopkins University. HeLa was used by Jonas Salk to test the polio vaccine in the 1950s and have been used extensively in research since then, having been used in over 60,000 publications to date. In March of 2013, the HeLa genome was sequenced.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.