Human Heart Tissue Lysate (Adult)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-25026
    • Availability
      Product Discontinued

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Applications/Dilutions

Application Notes
Each tissue or cell lysate is prepared in 1x sample buffer at a concentration ideal for most SDS-PAGE applications. One vial of this Lysate is sufficient to run 10 lanes (10 ug/lane) of protein. All tissues are extracted from adult humans. Use in Western blot reported in scientific literature (PMID 24253521)
Publications
Read Publications using
NBP2-25026 in the following applications:

  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at -80C in the dark. Avoid freeze-thaw cycles.
Buffer
HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40, MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors
Concentration
2 mg/ml

Lysate Details for Heart

Type
Tissue
Life Stage
Adult

Background

The identification of the human genome has promoted novel studies involving aging, behavioral research, diabetes and obesity, immunology, bioassay and pharmacological screening. Western blot analysis assists scientists conducting such research to make important contributions to functional genomics, allowing for structural, functional and comparative analysis of proteins in human models. Imgenex introduces cell lysates that provide a simple and fast solution for screening the expression of a particular protein in human tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Heart Lysate (NBP2-25026)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
All Applications
Filter By Species
Human
(1)
All Species
Showing Publications 1 - 4 of 4.
Publications using NBP2-25026 Applications Species
Peterfi Z, Toth ZE, Kovacs HA et al. Peroxidasin-like protein: a novel peroxidase homologue in the human heart. Cardiovasc. Res. 3/1/2014 [PMID: 24253521] (WB) WB
Roth W, Kermer P, Krajewska M et al. Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways. Cell Death Differ. 2003 Oct [PMID: 14502241] (WB, Human)

Details:
WB (Fig 1):human tissue lysates including Brain (40141), Heart (40142), Intestine (40143), kidney (40144), liver (40145), lung (40146), muscle (40147), spleen (40149), and testis (40150), SK-N-BE neuroblastoma cells.
WB Human
Dube S, Yalamanchili S, Lachant J et al. Expression of Tropomyosin 2 Gene Isoforms in Human Cardiac Tissue Int J Cardiol. 2014 Jun 25 (WB) WB
Dube S, Yalamanchili S, Lachant J, Abbott, L. Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines International Journal of Breast Cancer. 2015 Apr 28 (WB) WB

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Heart Lysate (NBP2-25026). (Showing 1 - 1 of 1 FAQs).

  1. Is SDS present in this product?
    • Our total proteins are already SDS free; therefore, you should not experience issues with SDS being present.

Additional Heart Products

Heart NBP2-25026

Blogs on Heart

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Calculators

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