HDGF Antibody

Western Blot: HDGF Antibody [NBP1-71926] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-HDGF antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies NBP1-71924 and NBP1-71925, which recognize upstream epitopes.

Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-HDGF left image, middle image and right image used at a dilution of 1: 1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HDGF Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 200 and 240 of human Hepatoma-Derived Growth Factor using the numbering given in entry NP_004485.1 (GeneID 3068).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDGF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000- 1:5000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HDGF Antibody

• FLJ96580
• HDGF
• hepatoma-derived growth factor (high-mobility group protein 1-like)
• hepatoma-derived growth factor
• High mobility group protein 1-like 2
• HMG-1L2
• HMG1L2DKFZp686J1764
• HuHGF

Background

HDGF is a member of the hepatoma-derived growth factor family. This protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HDGF Antibody (NBP1-71926)(1)

Publication using NBP1-71926

• G Jacquemin, A Wurmser, M Huyghe, W Sun, Z Homayed, C Merle, M Perkins, F Qasrawi, S Richon, F Dingli, G Arras, D Loew, D Vignjevic, J Pannequin, S Fre Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme Elife, 2022-05-11;11(0):. 2022-05-11 [PMID: 35543624]

Bioinformatics

• Gene Symbol: HDGF
• Uniprot:
  • NP_004485.1(Human)