HDAC7 Antibody

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Western Blot: HDAC7 Antibody [NB120-12175] - Mouse NIH 3T3 whole cell extracts were separated on SDS-PAGE, blotted with Rabbit Anti-HDAC7 (YS-19) at a 1:2,000 dilution and developed using Goat anti-Rabbit IgG-Peroxidase ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HDAC7 Antibody Summary

Immunogen
Synthetic peptide: YRTLEPLDTEGAARSVLSS with N-terminal added lysine, conjugated to KLH, corresponding to amino acids 137-155 of Mouse HDAC7. The corresponding sequence is identical in rat and differs by three amino acids in human.
Localization
Nuclear and cytoplasmic
Specificity
HDAC7
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC7
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunoprecipitation 1.5-1.8 ug/mg lysate
Application Notes
IP: Use at an assay dependent dilution. 1.5-1.8 ug of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 ug) of 293T human embryonic kidney cells expressing recombinant human HDAC7.WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa. Additional weak bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide. A minimum working antibody dilution of 1/2000 is recommended using whole extracts of mouse NIH-3T3 cells. A minimum working antibody dilution of 1/500 is recommended using whole extracts of rat PC12 cells.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4) and 1.0% BSA
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC7 Antibody

  • DKFZP586J0917
  • EC 3.5.1.98
  • HD7
  • HD7A
  • HDAC7AFLJ99588
  • histone deacetylase 7
  • Histone deacetylase 7ADKFZp586J0917

Background

HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HDAC7
Entrez
Uniprot