HDAC3 Antibody

Product Discontinued

HDAC3 Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human HDAC3. The immunogen region is: C-terminal.
• Specificity: HDAC3 (Ab-424) Antibody detects endogenous levels of total HDAC3 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000

Reactivity Notes

Human,Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HDAC3 Antibody

• EC 3.5.1.98
• HD3RPD3-2RPD3
• HDAC3
• Histone Deacetylase 3
• KDAC3
• RPD3-2
• SMAP45

Background

HDAC3 is a 50 kD class I histone deacetylase similar to yeast RPD3 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC3 is modified by phosphorylation and sumoylation. HDAC3 has been reported to interact with HDAC4, SMRT and N-CoR co-repressors, GATA-2 and TF-II-I. The Poly6076 antibody has been shown to be useful for Western blotting and immunofluorescence detection of human HDAC3. Interacts with HDAC4, SMRT and N-CoR co-repressors, GATA-2, and TF-II-I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC3
• Uniprot:
  • O15379(Human)