HDAC1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NB500-124] - HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Rabbit Anti-HDAC1. ...read more
Immunoprecipitation: HDAC1 Antibody [NB500-124] - 1. 5 uL antibody. 2. 10 uL antibody. 3. Negative control: 10 uL non-relevant antibody.
Immunoblotting: HDAC1 Antibody [NB500-124] - 1. HEK-293T; 2. HeLa; 3. K562; 4. CHO; 5. NIH-3T3; 6. PC-12

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB500-124
    • Availability
      Product Discontinued

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HDAC1 Antibody Summary

Immunogen
Synthetic peptide: C-KEEKPEAKGVKEEVKLA conjugated to KLH, corresponding to amino acids 466-482 of Human HDAC 1.
Epitope
aa 466-482
Localization
Nuclear
Specificity
Anti-Histone Deacetylase 1 specifically recognizes histone deacetylase 1 by immunoblotting and immunoprecipitation (65 kD).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:20000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:2500
Application Notes
This antibody can be used in Chromatin Immunoprecipitaion, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/Immunofluorescence, and Immunoprecipitation. For IHC perform enzymatic antigen retrieval before commencing with IHC staining protocol. In Western Blot a 55kDa band is seen.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB500-124.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
11.6 mg/ml
Purity
IgG purified

Alternate Names for HDAC1 Antibody

  • EC 3.5.1.98
  • GON-10
  • HD1DKFZp686H12203
  • histone deacetylase 1
  • reduced potassium dependency, yeast homolog-like 1
  • RPD3L1RPD3

Background

The basic repeating unit of chromatin is the nucleosome, which consists of 146 bp of DNA wound about a histone octamer composed of two each of the core histones H2A, H2B, H3 and H4. Reversible acetylation of highly conserved lysine residues in N-terminal tail domains of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. Several histone acetyltransferases (HATs) catalyze the acetylation reaction (GCN5, PCAF, p300/CBP, TAFII 250, P/CAF, SRC-1, BRCA-2). Acetylation of the core histones is generally considered to be associated with gene activation, probably through maintenance of the unfolded structure of transcribing nucleosomes. Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes. Other components of such complexes may include: corepressors such as mSin3, N-CoR, SMRT, associated proteins such as SAP18, SAP30, RbAp46, RbAp48, c-Ski oncogenic protein, a protein involved in DNA methylation etc. Nucleosome remodeling and deacetylation (NRD) complexes containing HDAC1, HDAC2, Mi-2 (CH3, CH4) dermatomyositis specific autoantigen and MTA2 protein that is related to metastasis-associated protein 1, were recently described. In this way, ATP-dependent nucleosome remodeling activity and histone deacetylation may be interconnected or interdependent. Recruitment of the multiprotein complexes to promoter sites occurs by many sequence specific DNA -binding proteins such as unliganded nuclear hormone receptors, DP1-E2F, YY1 and Rb family of transcription factors, transcriptional repressors and tumor suppressors (e.g. BRCA1). Aberrant recruitment of HDACs by certain oncoproteins may occur in certain neoplastic diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC1 Antibody (NB500-124)(13)

We have publications tested in 1 confirmed species: Human.


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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB500-124 Applications Species
Toda T, Hsu JY, Linker SB et al. Nup153 Interacts with Sox2 to Enable Bimodal Gene Regulation and Maintenance of Neural Progenitor Cells Cell Stem Cell 2017 Sep 13 [PMID: 28919367]
Opsahl JA, Hjornevik LV, Bull VH et al. Increased interaction between DJ-1 and the Mi-2/ nucleosome remodelling and deacetylase complex during cellular stress. Proteomics 2010 Apr [PMID: 20127688] (Human) Human
Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73. 1999 [PMID: 10220385]
Fischle W, Emiliani S, Hendzel MJ, Nagase T, Nomura N, Voelter W, Verdin E. General / background references, A new family of human histone deacetylases related to Saccharomyces cerevisiae HDA1p. J Biol Chem 274:11713-20. 1999 [PMID: 10206986]
Tong JK et al. Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex. Nature 395:917-21. 1998 [PMID: 9804427]
Zhang Y et al. The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase nucleosome remodeling activities. Cell 95:279-89. 1998 [PMID: 9790534]
Hassig CA et al. A role for histone deacetylase activity in HDAC1-mediated transcriptional repression. Proc Natl Acad Sci U S A 95:3519-24. 1998 [PMID: 9520398]
Lin RJ et al. Role of the histone deacetylase complex in acute promyelocytic leukaemia. Nature 391:811-4. 1998 [PMID: 9486654]
Grunstein M. Histone acetylation in chromatin structure transcription. Nature 389:349-52. 1997 [PMID: 9311776]
Taunton J et al. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 272:408-11. 1996 [PMID: 8602529]
Show All 13 Publications.

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ChIP Video Protocol
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ICC/IF Video Protocol

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Bioinformatics Tool for HDAC1 Antibody (NB500-124)

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Research Areas for HDAC1 Antibody (NB500-124)

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Blogs on HDAC1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol HDAC1
Entrez