HCST Overexpression Lysate (Native)

Images

 
Western Blot: HCST Overexpression Lysate (Adult Normal) [NBL1-11476] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCST.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HCST Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219253 and based on accession number NM_001007469. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 2, mRNA.
Gene
HCST

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
7.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HCST

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HCST Overexpression Lysate (Native)

  • DAP10PIK3AP
  • DKFZP586C1522
  • hematopoietic cell signal transducer
  • KAP10DNAX-activation protein 10
  • kinase assoc pro of ~10kDa
  • kinase assoc protein
  • Membrane protein DAP10
  • phosphoinositide-3-kinase adaptor protein
  • Transmembrane adapter protein KAP10

Background

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HCST Lysate (NBL1-11476) (0)

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Video Protocols

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Bioinformatics

Gene Symbol HCST