HCST Overexpression Lysate (Native)


Western Blot: HCST Overexpression Lysate (Adult Normal) [NBL1-11476] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HCST.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

HCST Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC219253 and based on accession number NM_001007469. The protein contains a C-terminal DDK tag.
Homo sapiens hematopoietic cell signal transducer (HCST), transcript variant 2, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
7.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for HCST

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HCST Overexpression Lysate (Native)

  • DKFZP586C1522
  • hematopoietic cell signal transducer
  • KAP10DNAX-activation protein 10
  • kinase assoc pro of ~10kDa
  • kinase assoc protein
  • Membrane protein DAP10
  • phosphoinositide-3-kinase adaptor protein
  • Transmembrane adapter protein KAP10


This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HCST Lysate (NBL1-11476) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Additional HCST Overexpression Lysate (Native) Products

HCST NBL1-11476

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Blogs on HCST

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Contact Information

Product PDFs


Gene Symbol HCST

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