HAUS8 Antibody

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Western Blot: HAUS8 Antibody [NBP1-77746] - Analysis shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HAUS8. In right lane ...read more

Product Details

Summary
Reactivity Hu, Or, PmSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HAUS8 Antibody Summary

Immunogen
Anti-HAUS8 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length HAUS8 protein.
Specificity
This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology.
Predicted Species
Orangutan (90%), Primate (90%). Backed by our 100% Guarantee.
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
HAUS8
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • ELISA 1:250000
Application Notes
Specific conditions for reactivity and detection of HAUS8 should be optimized by the end user. Expect a band approximately ~45 kDa in size corresponding - HAUS8 by Western Blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive in Human. Expected reactivity with HAUS8 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HAUS8 Antibody

  • DGT4
  • HAUS Augmin Like Complex Subunit 8
  • HEC1/NDC80 interacting, centrosome associated 1
  • HEC1/NDC80-Interacting Centrosome-Associated Protein 1
  • Hec1-Interacting And Centrosome-Associated 1
  • HICE1
  • NY-SAR-48
  • Sarcoma Antigen NY-SAR-48

Background

HAUS8 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the 'TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HAUS8
Uniprot