Haptoglobin Antibody (4H5)

Images

 
Western Blot: Haptoglobin Antibody (4H5) [NBP2-03008] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Haptoglobin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: Haptoglobin Antibody (4H5) [NBP2-03008] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Haptoglobin.
Flow Cytometry: Haptoglobin Antibody (4H5) [NBP2-03008] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Haptoglobin antibody, and ...read more
Western Blot: Haptoglobin Antibody (4H5) [NBP2-03008] Analysis of extracts (35ug) from 9 different cell lines by using anti-Haptoglobin monoclonal antibody.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
4H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Haptoglobin Antibody (4H5) Summary

Immunogen
Full length human recombinant protein of human HP (NP_005134) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HP
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Haptoglobin Antibody (4H5)

  • binding peptide
  • BP
  • haptoglobin alpha(1S)-beta
  • haptoglobin alpha(2FS)-beta
  • Haptoglobin
  • haptoglobin, alpha polypeptide
  • haptoglobin, beta polypeptide
  • HP
  • HP-1
  • hp2-alpha
  • HP2ALPHA2
  • HPA1S
  • MGC111141

Background

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HP
Entrez