HAND1 Antibody (1G10)

Images

 
Western Blot: HAND1 Antibody (1G10) [NBP2-00576] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: ...read more
Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (1G10) [NBP2-00576] Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.
Flow Cytometry: HAND1 Antibody (1G10) [NBP2-00576] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: HAND1 Antibody (1G10) [NBP2-00576] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Flow Cytometry: HAND1 Antibody (1G10) [NBP2-00576] - Analysis of Hela cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
1G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HAND1 Antibody (1G10) Summary

Immunogen
Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HAND1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HAND1 Antibody (1G10)

  • BHLHA27
  • bHLHa27heart- and neural crest derivatives-expressed protein 1
  • Class A basic helix-loop-helix protein 27
  • eHand
  • Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1
  • HAND1
  • heart and neural crest derivatives expressed 1
  • Hxt
  • Thing1

Background

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HAND1
Entrez