HA Tag Antibody [Agarose Immobilized]

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Product Details

Summary
Reactivity Hu, Ec, NASpecies Glossary
Applications IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Agarose Immobilized

HA Tag Antibody [Agarose Immobilized] Summary

Description
0.1 mg antibody/0.4 ml of 50% gel slurry
Immunogen
Rabbits were immunized with HA cleavage site (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation
Application Notes
Prepare working dilutions immediately before use. Chromatin Immunoprecipitation and Immunohistochemistry were reported in scientific literature. Immunoprecipitation 15 to 25 mcl of gel slurry per 0.1 to 1 mg of protein lysate or extract

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27203423).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HA Tag Antibody [Agarose Immobilized]

  • HA Epitope Tag
  • HA Tag
  • HA
  • Hemagglutinin

Background

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA Tag Antibody (NBP1-30434) (0)

There are no publications for HA Tag Antibody (NBP1-30434).
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Product General Protocols

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FAQs for HA Tag Antibody (NBP1-30434). (Showing 1 - 1 of 1 FAQ).

  1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
    • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB600-363B
FITC Labeled NB600-363F
HRP Labeled NB600-391

Additional HA Tag Products

HA Tag NBP1-30434

Research Areas for HA Tag Antibody (NBP1-30434)

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