H4/d Overexpression Lysate (Native)

Images

 
Western Blot: H4/d Overexpression Lysate (Adult Normal) [NBP2-10888] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H4/d.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

H4/d Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_003530. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens H4/d, mRNA.
Gene
HIST1H4K

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
11.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for H4/d

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for H4/d Overexpression Lysate (Native)

  • dJ160A22.1
  • H4/d
  • H4F2iii
  • H4FD
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST2H4A
  • HIST2H4B
  • histone 1, H4k
  • histone cluster 1, H4k
  • histone H4
  • member D

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-171
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NB100-56340
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
NBP1-58095
Species: Hu
Applications: WB
NB100-56346
Species: Hu, Mu
Applications: WB, ELISA, IHC-P
H00008349-M06
Species: Hu
Applications: ELISA, IHC-P
NB100-616
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
NBP1-91269
Species: Hu, Mu
Applications: ICC/IF (-), IHC (-), WB
H00007001-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-1923
Species: Ce
Applications: WB, IHC, IHC-P, IP
NB100-381
Species: Hu
Applications: WB, ChIP, IP, PLA
NBP1-87109
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1669
Species: Hu
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
NB100-55251
Species: Hu, Mu
Applications: WB, IP
NBP2-24613
Species: Hu
Applications: WB, Simple Western, IHC-P
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP1-87425
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P

Publications for H4/d Lysate (NBP2-10888) (0)

There are no publications for H4/d Lysate (NBP2-10888).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for H4/d Lysate (NBP2-10888) (0)

There are no reviews for H4/d Lysate (NBP2-10888). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for H4/d Lysate (NBP2-10888) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional H4/d Products

H4/d NBP2-10888

Bioinformatics Tool for H4/d Lysate (NBP2-10888)

Discover related pathways, diseases and genes to H4/d Lysate (NBP2-10888). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for H4/d Lysate (NBP2-10888)

Discover more about diseases related to H4/d Lysate (NBP2-10888).
 

Pathways for H4/d Lysate (NBP2-10888)

View related products by pathway.

PTMs for H4/d Lysate (NBP2-10888)

Learn more about PTMs related to H4/d Lysate (NBP2-10888).

Blogs on H4/d

There are no specific blogs for H4/d, but you can read our latest blog posts.

Customers Who Bought This Also Bought

HDAC1 Antibody
NB100-56340

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our H4/d Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol HIST1H4K