Recombinant Human H2AX His Protein

Images

 
Western Blot: H2AX Protein [NBP1-42464] - The anti-H2AX antibody NB100-638 was used to detect this protein. 200ng of protein was loaded onto the gel and NB100-638 was used to detect the protein.
SDS-Page: H2AX Protein [NBP1-42464] - This H2AX protein was tested on a 4-20% SDS-PAGE gel with 5ug of protein loaded on the gel. The purity of the protein is >95%.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human H2AX His Protein Summary

Description
A full-length recombinant protein corresponding to amino acids 2 - 143 of H2AX. Accession #: P16104.

Source: E. coli

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
H2AFX
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
This product is useful as a positive control for both Western Blot and SDS-Page.
Theoretical MW
17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
27 mM Tris pH 8.0, 250 mM NaCl, 8 M Urea, 1 mM DTT
Preservative
N/A
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human H2AX His Protein

  • BTF antibHistone H2AX
  • Gamma H2AX
  • H2A histone family member X
  • H2A.FX
  • H2A.X
  • H2A/X
  • H2AFX
  • H2AX histone
  • H2AX
  • Hist5.2ax
  • Histone 2A
  • Histone 2A.X
  • Histone 2AX
  • Histone H2A.x
  • Histone H2A/X
  • Histone H2AFX
  • Histone H2AX
  • Including osteosarcoma
  • OSRC
  • P105 RB
  • P105RB
  • PP105
  • PP110
  • pRb
  • RB 1
  • RB1 protein
  • RB1
  • Retinoblastoma 1 (including osteosarcoma)
  • Retinoblastoma 1
  • Retinoblastoma associated protein
  • Retinoblastoma related osteosarcoma
  • Retinoblastoma susceptibility gene

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Human Histone H2AX with an N-terminal His tag expressed in E. coli and purified by conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for H2AX Recombinant Protein (NBP1-42464). (Showing 1 - 1 of 1 FAQ).

  1. I am doing Western blot for H2AX using HL-60 cells, but I do not know which secondary antibody should I use, that is rabbit, mouse or goat?
    • Our lab recommends using a secondary that is appropriate to the primary antibody chosen for your Westerns. Therefore, if you decide to choose a Rabbit polyclonal anti-H2AX antibody, we would recommend an anti-Rabbit IgG secondary conjugated to HRP, or your specific detection system. The host of the secondary should not make a difference in your western blot assay.

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Bioinformatics

Gene Symbol H2AFX