H2AX Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

H2AX Antibody Summary

Immunogen
Synthetic peptide derived from C term part of the human H2a/x protein.
Specificity
Antiserum reacts with the 15 kDa H2a/x protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AFX
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Publications
Read Publication using NBP1-49584.

Reactivity Notes

Human, may cross react with H2A phosphoserine-140 and with bovine, mouse and rat proteins.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Antiserum
Preservative
No Preservative
Purity
Unpurified

Alternate Names for H2AX Antibody

  • BTF antibHistone H2AX
  • Gamma H2AX
  • H2A histone family member X
  • H2A.FX
  • H2A.X
  • H2A/X
  • H2AFX
  • H2AX histone
  • H2AX
  • Hist5.2ax
  • Histone 2A
  • Histone 2A.X
  • Histone 2AX
  • Histone H2A.x
  • Histone H2A/X
  • Histone H2AFX
  • Histone H2AX
  • Including osteosarcoma
  • OSRC
  • P105 RB
  • P105RB
  • PP105
  • PP110
  • pRb
  • RB 1
  • RB1 protein
  • RB1
  • Retinoblastoma 1 (including osteosarcoma)
  • Retinoblastoma 1
  • Retinoblastoma associated protein
  • Retinoblastoma related osteosarcoma
  • Retinoblastoma susceptibility gene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for H2AX Antibody (NBP1-49584)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for H2AX Antibody (NBP1-49584). (Showing 1 - 1 of 1 FAQ).

  1. I am doing Western blot for H2AX using HL-60 cells, but I do not know which secondary antibody should I use, that is rabbit, mouse or goat?
    • Our lab recommends using a secondary that is appropriate to the primary antibody chosen for your Westerns. Therefore, if you decide to choose a Rabbit polyclonal anti-H2AX antibody, we would recommend an anti-Rabbit IgG secondary conjugated to HRP, or your specific detection system. The host of the secondary should not make a difference in your western blot assay.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for H2AX Antibody (NBP1-49584)

Discover related pathways, diseases and genes to H2AX Antibody (NBP1-49584). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on H2AX.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

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Bioinformatics

Gene Symbol H2AFX