H2AX Antibody

Images

 
Western Blot: H2AX Antibody [NB100-78355] - Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-H2A.X antibody. Proteins were visualized using a donkey ...read more
Immunocytochemistry/ Immunofluorescence: H2AX Antibody [NB100-78355] - la cells were fixed in 4% paraformaldehyde, washed in PBS, and permeabilized using 0.5% Triton X-100 for 5 minutes. Cells were then incubated with a ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78355
    • Availability
      Product Discontinued

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H2AX Antibody Summary

Immunogen
Recominant (partial), N-terminal
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AFX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
gamma H2AX Lysate (NBL1-11424)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for H2AX Antibody

  • BTF antibHistone H2AX
  • Gamma H2AX
  • H2A histone family member X
  • H2A.FX
  • H2A.X
  • H2A/X
  • H2AFX
  • H2AX histone
  • H2AX
  • Hist5.2ax
  • Histone 2A
  • Histone 2A.X
  • Histone 2AX
  • Histone H2A.x
  • Histone H2A/X
  • Histone H2AFX
  • Histone H2AX
  • Including osteosarcoma
  • OSRC
  • P105 RB
  • P105RB
  • PP105
  • PP110
  • pRb
  • RB 1
  • RB1 protein
  • RB1
  • Retinoblastoma 1 (including osteosarcoma)
  • Retinoblastoma 1
  • Retinoblastoma associated protein
  • Retinoblastoma related osteosarcoma
  • Retinoblastoma susceptibility gene

Background

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The Poly6133 antibody has been shown to be useful for Western blotting and immunofluorescence of human H2A.X.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for H2AX Antibody (NB100-78355). (Showing 1 - 1 of 1 FAQ).

  1. I am doing Western blot for H2AX using HL-60 cells, but I do not know which secondary antibody should I use, that is rabbit, mouse or goat?
    • Our lab recommends using a secondary that is appropriate to the primary antibody chosen for your Westerns. Therefore, if you decide to choose a Rabbit polyclonal anti-H2AX antibody, we would recommend an anti-Rabbit IgG secondary conjugated to HRP, or your specific detection system. The host of the secondary should not make a difference in your western blot assay.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional H2AX Products

Bioinformatics Tool for H2AX Antibody (NB100-78355)

Discover related pathways, diseases and genes to H2AX Antibody (NB100-78355). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for H2AX Antibody (NB100-78355)

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Pathways for H2AX Antibody (NB100-78355)

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Research Areas for H2AX Antibody (NB100-78355)

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Blogs on H2AX.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

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Bioinformatics

Gene Symbol H2AFX
Entrez
Uniprot