Recombinant Human Growth Hormone 2 Protein

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SDS-Page: GH2 Protein [NBC1-18451] - 20 kDa Growth hormone, 20 kDa (177 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Growth Hormone 2 Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 27-202 of Human Growth Hormone 2

Source: E.coli

Amino Acid Sequence: MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF

Details of Functionality
Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 for this effect is less or equal to 1ng/ml.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
GH2
Purity
>95% pure by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate Buffer Saline (pH 7.4) containing 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Growth Hormone 2 Protein

  • GH2
  • GHL
  • GHV
  • GHVgrowth hormone variant
  • GH-VPlacenta-specific growth hormone
  • Growth Hormone 2
  • growth hormone 2hGH-V
  • placental-specific growth hormone

Background

The 20KDa variant form of human growth hormone (20KDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22K, 191 amino acid) by the deletion of amino acid residues 32-46. The physiological role of 20K hGH remains to be determined partly but it regards the main function as stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant 20KDa human growth hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol GH2
Entrez