GRASP65 Antibody (Poly6210)

Images

 
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (Poly6210) [NB100-78413] - Vero E6 cells stained with purified rabbit anti-GRASP65, followed by Cy3-conjugated goat anti-rabbit IgG and DAPI.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (Poly6210) [NB100-78413] - A549 cells stained with purified rabbit anti-GRASP65, followed by Cy3-conjugated goat anti-rabbit IgG and DAPI.

Product Details

Summary
Product Discontinued
View other related GRASP65 Primary Antibodies

Order Details


    • Catalog Number
      NB100-78413
    • Availability
      Product Discontinued

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GRASP65 Antibody (Poly6210) Summary

Immunogen
Recombinant (partial)
Specificity
GRASP65
Clonality
Monoclonal
Host
Rabbit
Gene
GORASP1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:400
Application Notes
Immunofluorescence staining, suggested working dilution(s): 1:400 dilution.
Positive Control
GRASP65 Lysate (NBL1-11202)

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for GRASP65 Antibody (Poly6210)

  • Golgi peripheral membrane protein p65
  • Golgi phosphoprotein 5MGC118897
  • golgi reassembly stacking protein 1, 65kDa
  • Golgi reassembly-stacking protein 1
  • Golgi reassembly-stacking protein of 65 kDa
  • GRASP65MGC118894
  • P6565 kDa

Background

GRASP65 (golgi reassembly stacking protein 1, Golgi peripheral membrane protein p65, reassembly and stacking protein p65) is a 65 kD Golgi stacking protein. Alternatively spliced transcripts of GRASP65 have been identified. This protein is thought to play a role in establishing the stacked structure of the Golgi cisternae allowing sorting and modification of proteins exported from the endoplasmic reticulum. GRASP65 is cleaved by caspase-3 during apoptosis and can be partitioned by fragmentation and dispersal during entry into mitosis. GRASP65 is modified by phosphorylation (polo-like kinase-1 and cdc2-cyclin B). This protein has been shown to form a complex with the Golgi matrix protein GOLGA2; this complex then interacts with the vesicle docking protein p115,and tethering factor GM130.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GORASP1
Entrez
Uniprot