Granulocytes Antibody (BM-2) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
BM-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Granulocytes Antibody (BM-2) - Azide and BSA Free Summary

Immunogen
Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this granulocyte antibody.
Localization
Cytoplasmic
Specificity
Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. MAb BM-2 reacts with early precursor and mature forms of human myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSF3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • CyTOF-ready
Application Notes
No special pretreatment is required for staining of formalin/paraffin tissues. Use in Immunohistochemistry-Frozen has been reported in literature.

Reactivity Notes

Macaque Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Granulocytes Antibody (BM-2) - Azide and BSA Free

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3OS
  • Filgrastim
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • pluripoietin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CSF3