gp96/HSP90B1/GRP94 Antibody

Images

 
Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-19527] - Extracts from PC12 cells.
Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-19527] - Extracts from 293 cells.

Product Details

Summary
Product Discontinued
View other related gp96/HSP90B1/GRP94 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-19527
    • Availability
      Product Discontinued

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gp96/HSP90B1/GRP94 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 191-240 of Human HSP90B.
Marker
ER Stress Marker
Clonality
Polyclonal
Host
Rabbit
Gene
HSP90B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of HSP90B (E222) pAb in extracts from PC12 cells.
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for gp96/HSP90B1/GRP94 Antibody

  • 94 kDa glucose-regulated protein
  • ECGP
  • Endoplasmin
  • endothelial cell (HBMEC) glycoprotein
  • glucose regulated protein, 94 kDa
  • gp96
  • GP96ECGP
  • Grp94
  • GRP-94
  • GRP94gp96 homolog
  • Heat shock protein 90 kDa beta member 1
  • heat shock protein 90kDa beta (Grp94), member 1
  • HSP90B1
  • TRA1endoplasmin
  • tumor rejection antigen (gp96) 1
  • Tumor rejection antigen 1
  • Tumor rejection antigen-1 (gp96)

Background

The 90-kDa heat shock protein (Hsp90) is a highly conserved, and abundant cytosolic homodimeric molecular chaperone. Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine and serine/threonine kinases and actin. Two isoforms which correspond to the major and minor isoform, (Hsp90 alpha and Hsp90 beta) can be found in nearly equal amount in humans, and operates as part of a multichaperone machinery in the cytosol, which includes Hsp70, peptidyl-prolyl isomerases and other cochaperones.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for gp96/HSP90B1/GRP94 Antibody (NBP1-19527)

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Diseases for gp96/HSP90B1/GRP94 Antibody (NBP1-19527)

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Bioinformatics

Gene Symbol HSP90B1
Entrez