GOLGA3 Antibody

Western Blot: GOLGA3 Antibody [NB100-2442] - Staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.1ug/ml)

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GOLGA3 Antibody Summary

• Description: Novus Biologicals Goat GOLGA3 Antibody (NB100-2442) is a polyclonal antibody validated for use in WB and ELISA. Anti-GOLGA3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HSQSRASKEGPGE corresponding to C-Terminus according to NP_005886.2.
• Localization: Cytoplasmic and Golgi apparatus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GOLGA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 170 kDa band observed in human HeLa lysates (calculated MW of 167 kDa band according to NP_005886.2). An additional band of unknown identity was also consistently observed at approx. 25 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  HeLa Whole Cell Lysate

• Control Peptide:

  GOLGA3 Peptide (NB100-2442PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GOLGA3 Antibody

• GCP170Golgi peripheral membrane protein
• golgi autoantigen, golgin subfamily a, 3
• Golgi complex-associated protein of 170 kD
• Golgi complex-associated protein of 170 kDa
• Golgi membrane associated protein
• golgin A3
• Golgin subfamily A member 3
• golgin-160
• golgin-165
• male enhanced antigen-2
• MEA-2
• SY2/SY10 protein

Background

GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOLGA3 Antibody (NB100-2442)(1)

Publication using NB100-2442

• Hicks SW, Machamer CE. The NH2-terminal domain of Golgin-160 contains both Golgi and nuclear targeting information. J Biol Chem 2002-09-27 [PMID: 12130652]

Bioinformatics

• Gene Symbol: GOLGA3
• Entrez:
  • 2802(Human)
• Uniprot:
  • NP_005886.2(Human)